PDBe 1ml0

X-ray diffraction
2.8Å resolution

VIRAL CHEMOKINE BINDING PROTEIN M3 FROM MURINE GAMMAHERPESVIRUS68 IN COMPLEX WITH THE P8A VARIANT OF CC-CHEMOKINE MCP-1

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero 12-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
M3 protein Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 41.83 KDa
Source organism: Murid gammaherpesvirus 4
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O41925 (Residues: 25-406; Coverage: 94%)
Gene names: GAMMAHV.M3, M3
Sequence domains: M3
Structure domains:
C-C motif chemokine 2 Chain: D
Molecule details ›
Chain: D
Length: 76 amino acids
Theoretical weight: 8.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13500 (Residues: 24-99; Coverage: 100%)
Gene names: CCL2, MCP1, SCYA2
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P321
Unit cell:
a: 99.2Å b: 99.2Å c: 78.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.282
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli