PDBe 1mki

X-ray diffraction
2Å resolution

Crystal Structure of Bacillus Subtilis Probable Glutaminase, APC1040

Released:

Function and Biology Details

Reaction catalysed:
L-glutamine + H(2)O = L-glutamate + NH(3). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31465 (Residues: 1-327; Coverage: 100%)
Gene names: BSU02430, glsA, glsA1, ybgJ
Sequence domains: Glutaminase
Structure domains:

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 71.332Å b: 181.482Å c: 51.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.245
Expression system: Escherichia coli BL21(DE3)