PDBe 1mhy

X-ray diffraction
2Å resolution

METHANE MONOOXYGENASE HYDROXYLASE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Methane monooxygenase component A beta chain Chain: B
Molecule details ›
Chain: B
Length: 395 amino acids
Theoretical weight: 45.17 KDa
Source organism: Methylosinus trichosporium
UniProt:
  • Canonical: P27354 (Residues: 1-347, 348-368, 371-371, 373-394; Coverage: 98%)
Gene name: mmoY
Sequence domains: Methane/Phenol/Toluene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Methane monooxygenase component A alpha chain Chain: D
Molecule details ›
Chain: D
Length: 521 amino acids
Theoretical weight: 59.51 KDa
Source organism: Methylosinus trichosporium
UniProt:
  • Canonical: P27353 (Residues: 1-328, 330-526; Coverage: 99%)
Gene name: mmoX
Sequence domains: Methane/Phenol/Toluene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Methane monooxygenase component A gamma chain Chain: G
Molecule details ›
Chain: G
Length: 169 amino acids
Theoretical weight: 19.36 KDa
Source organism: Methylosinus trichosporium
UniProt:
  • Canonical: P27355 (Residues: 1-169; Coverage: 100%)
Gene name: mmoZ
Sequence domains: Methane monooxygenase, hydrolase gamma chain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 264.49Å b: 71.19Å c: 139.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.137 not available