› 1mh1

X-ray diffraction
1.38Å resolution

SMALL G-PROTEIN

Released:
DOI: 10.2210/pdb1mh1/pdb
PDB entry 1mh1 coloured by chain, front view.
PDB entry 1mh1 coloured by chain, side view.
PDB entry 1mh1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue.
Hirshberg M, Stockley RW, Dodson G, Webb MR
Nat Struct Biol 4 147-52 (1997)
PMID: 9033596

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 2-184; Coverage: 95%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21212
Unit cell:
a: 46.44Å b: 103.05Å c: 43.673Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.205
Expression system: Escherichia coli

Quick links

Citations

17 review citations

GDIs: central regulatory molecules in Rho GTPase activation.
DerMardirossian et al. (2005)
16 more

48 mentions without citation

Rac1 as a Target to Treat Dysfunctions and Cancer of the Bladder.
Sauzeau et al. (2022)
47 more