Function and Biology

Crystal structure of Drosophila melanogaster alcohol dehydrogenase complexed with NADH and acetate at 1.6 A

Source organism: Drosophila melanogaster
Biochemical function: oxidoreductase activity
Biological process: oxidation-reduction process
Cellular component: protein complex

EC 1.1.1.1: Alcohol dehydrogenase

Reaction catalysed:
(1) A primary alcohol + NAD(+) = an aldehyde + NADH. (2) A secondary alcohol + NAD(+) = a ketone + NADH.
Comments:
  • Acts on primary or secondary alcohols or hemi-acetals with very broad specificity; however the enzyme oxidizes methanol much more poorly than ethanol.
  • The animal, but not the yeast, enzyme acts also on cyclic secondary alcohols.
Systematic name:
Alcohol:NAD(+) oxidoreductase
Alternative Name(s):
  • ADH
  • NAD-dependent alcohol dehydrogenase
  • NAD-specific aromatic alcohol dehydrogenase
  • NADH-alcohol dehydrogenase
  • NADH-aldehyde dehydrogenase
  • Alcohol dehydrogenase (NAD)
  • Aldehyde reductase
  • Aliphatic alcohol dehydrogenase
  • Ethanol dehydrogenase
  • Primary alcohol dehydrogenase
  • Yeast alcohol dehydrogenase

Sequence families

Pfam Protein families (Pfam)
PF00106
Domain description: short chain dehydrogenase
Occurring in:
  1. Alcohol dehydrogenase
1 copy of Pfam domain PF00106 (short chain dehydrogenase) in Alcohol dehydrogenase in PDB 1mg5.

PF08659
Domain description: KR domain
Occurring in:
  1. Alcohol dehydrogenase
1 copy of Pfam domain PF08659 (KR domain) in Alcohol dehydrogenase in PDB 1mg5.

PF13561
Domain description: Enoyl-(Acyl carrier protein) reductase
Occurring in:
  1. Alcohol dehydrogenase
1 copy of Pfam domain PF13561 (Enoyl-(Acyl carrier protein) reductase) in Alcohol dehydrogenase in PDB 1mg5.

InterPro InterPro annotations
IPR002424
Domain description: Alcohol dehydrogenase, insect-type
Occurring in:
  1. Alcohol dehydrogenase
IPR002425
Domain description: Alcohol dehydrogenase, Drosophila-type
Occurring in:
  1. Alcohol dehydrogenase
IPR002347
Domain description: Short-chain dehydrogenase/reductase SDR
Occurring in:
  1. Alcohol dehydrogenase
IPR036291
Domain description: NAD(P)-binding domain superfamily
Occurring in:
  1. Alcohol dehydrogenase
IPR020904
Domain description: Short-chain dehydrogenase/reductase, conserved site
Occurring in:
  1. Alcohol dehydrogenase

Structure domain

CATH CATH domain
3.40.50.720
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: NAD(P)-binding Rossmann-like Domain
Occurring in:
  1. Alcohol dehydrogenase
1 copy of CATH domain 3.40.50.720 (Rossmann fold) in Alcohol dehydrogenase in PDB 1mg5.
SCOP SCOP annotation
51751
Class: Alpha and beta proteins (a/b)
Fold: NAD(P)-binding Rossmann-fold domains
Superfamily: NAD(P)-binding Rossmann-fold domains
Occurring in:
  1. Alcohol dehydrogenase
1 copy of SCOP domain 51751 (Tyrosine-dependent oxidoreductases) in Alcohol dehydrogenase in PDB 1mg5.