PDBe 1mfp

X-ray diffraction
2.33Å resolution

E. coli Enoyl Reductase in complex with NAD and SB611113

Released:

Function and Biology Details

Reaction catalysed:
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl- carrier protein] + NADH. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 27.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEK4 (Residues: 1-262; Coverage: 100%)
Gene names: JW1281, b1288, envM, fabI
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6122
Unit cell:
a: 79.6Å b: 79.6Å c: 325.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.259
Expression system: Escherichia coli