PDBe 1mf8

X-ray diffraction
3.1Å resolution

Crystal Structure of human calcineurin complexed with cyclosporin A and human cyclophilin

Released:
Primary publication:
Crystal structure of human calcineurin complexed with cyclosporin A and human cyclophilin.
Proc. Natl. Acad. Sci. U.S.A. 99 13522-6 (2002)
PMID: 12357034

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CYCLOSPORIN A Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Tolypocladium inflatum
Expression system: Not provided
Calcineurin subunit B type 1 Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 19.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63098 (Residues: 1-170; Coverage: 100%)
Gene names: CNA2, CNB, PPP3R1
Sequence domains:
Structure domains: EF-hand
Peptidyl-prolyl cis-trans isomerase A Chain: C
Molecule details ›
Chain: C
Length: 165 amino acids
Theoretical weight: 18.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 42.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08209 (Residues: 20-392; Coverage: 72%)
Gene names: CALNA, CNA, PPP3CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 64.949Å b: 108.334Å c: 112.841Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.3
Expression systems:
  • Not provided
  • Escherichia coli