Function and Biology

BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP

Source organism: Streptomyces clavuligerus
Biochemical function: metal ion binding
Biological process: clavulanic acid biosynthetic process
Cellular component: not assigned

EC 6.3.3.4: (Carboxyethyl)arginine beta-lactam-synthase

Reaction catalysed:
ATP + L-N(2)-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate.
Comments:
  • Forms part of the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus.
  • It has been proposed that L-N(2)-(2-carboxyethyl)arginine is first converted into an acyl-AMP by reaction with ATP and loss of diphosphate, and that the beta-lactam ring is then formed by the intramolecular attack of the beta-nitrogen on the activated carboxy group.
Systematic name:
L-N(2)-(2-carboxyethyl)arginine cyclo-ligase (AMP-forming)
Alternative Name(s):
  • Beta-lactam synthetase
  • L-2-N-(2-carboxyethyl)arginine cyclo-ligase (AMP-forming)

Sequence families

CATH Protein families (Pfam)
PF00733
Domain description: Asparagine synthase
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
2 copies of Pfam domain PF00733 (Asparagine synthase) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.

PF13537
Domain description: Glutamine amidotransferase domain
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
1 copy of Pfam domain PF13537 (Glutamine amidotransferase domain) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.

CATH InterPro annotations
IPR001962
Domain description: Asparagine synthase
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
IPR029055
Domain description: Nucleophile aminohydrolases, N-terminal
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
IPR017932
Domain description: Glutamine amidotransferase type 2 domain
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
IPR014729
Domain description: Rossmann-like alpha/beta/alpha sandwich fold
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase

Structure domains

CATH CATH domains
3.40.50.620
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: HUPs
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
1 copy of CATH domain 3.40.50.620 (Rossmann fold) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.
3.60.20.10
Class: Alpha Beta
Architecture: 4-Layer Sandwich
Topology: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Homology: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
1 copy of CATH domain 3.60.20.10 (Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.
SCOP SCOP annotations
56236
Class: Alpha and beta proteins (a+b)
Fold: Ntn hydrolase-like
Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
1 copy of SCOP domain 56236 (Class II glutamine amidotransferases) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.
52403
Class: Alpha and beta proteins (a/b)
Fold: Adenine nucleotide alpha hydrolase-like
Superfamily: Adenine nucleotide alpha hydrolases-like
Occurring in:
  1. Carboxyethyl-arginine beta-lactam-synthase
1 copy of SCOP domain 52403 (N-type ATP pyrophosphatases) in Carboxyethyl-arginine beta-lactam-synthase in PDB 1mb9.