PDBe 1mb9

X-ray diffraction
2.11Å resolution

BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP

Released:
Source organism: Streptomyces clavuligerus

Function and Biology Details

Reaction catalysed:
ATP + L-N(2)-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxyethyl-arginine beta-lactam-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 54.6 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DJQ7 (Residues: 1-513; Coverage: 100%)
Gene name: bls
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 61.252Å b: 97.571Å c: 81.313Å
α: 90° β: 90.69° γ: 90°
R-values:
R R work R free
0.21 0.21 0.251
Expression system: Escherichia coli BL21(DE3)