PDBe 1m6d

X-ray diffraction
1.7Å resolution

Crystal structure of human cathepsin F


Function and Biology Details

Reaction catalysed:
The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (k(cat)/K(m)) comparable to that of cathepsin L. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Cathepsin F Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
  • Canonical: Q9UBX1 (Residues: 271-484; Coverage: 46%)
Gene name: CTSF
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 68.25Å b: 103.26Å c: 58.65Å
α: 90° β: 90° γ: 90°
R R work R free
0.198 0.195 0.222
Expression system: Komagataella pastoris