PDBe 1m63

X-ray diffraction
2.8Å resolution

Crystal structure of calcineurin-cyclophilin-cyclosporin shows common but distinct recognition of immunophilin-drug complexes

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CYCLOSPORIN A Chains: D, H
Molecule details ›
Chains: D, H
Length: 11 amino acids
Theoretical weight: 1.22 KDa
Source organism: Tolypocladium inflatum
Expression system: Not provided
Calcineurin subunit B type 1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 169 amino acids
Theoretical weight: 19.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63098 (Residues: 2-170; Coverage: 99%)
Gene names: CNA2, CNB, PPP3R1
Sequence domains:
Structure domains: EF-hand
Peptidyl-prolyl cis-trans isomerase A Chains: C, G
Molecule details ›
Chains: C, G
Length: 165 amino acids
Theoretical weight: 18.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Chains: A, E
Molecule details ›
Chains: A, E
Length: 372 amino acids
Theoretical weight: 42.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08209 (Residues: 1-372; Coverage: 71%)
Gene names: CALNA, CNA, PPP3CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P41212
Unit cell:
a: 108.657Å b: 108.657Å c: 316.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.322
Expression systems:
  • Not provided
  • Escherichia coli BL21