PDBe 1m61

X-ray diffraction
2.5Å resolution

Crystal structure of the apo SH2 domains of ZAP-70

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43403 (Residues: 1-256; Coverage: 41%)
Gene names: SRK, ZAP70
Sequence domains: SH2 domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 48.851Å b: 52.227Å c: 54.455Å
α: 90° β: 89.91° γ: 90°
R-values:
R R work R free
0.245 0.245 0.286
Expression system: Escherichia coli BL21(DE3)