› 1m5u

X-ray diffraction
1.87Å resolution

CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK. STRUCTURE AT PH 8.0 IN THE APO-FORM

Released:
DOI: 10.2210/pdb1m5u/pdb
PDB entry 1m5u coloured by chain, front view.
PDB entry 1m5u coloured by chain, side view.
PDB entry 1m5u coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
Crystallographic and biochemical studies of DivK reveal novel features of an essential response regulator in Caulobacter crescentus.
Guillet V, Ohta N, Cabantous S, Newton A, Samama JP
J Biol Chem 277 42003-10 (2002)
PMID: 12176983

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.06 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A5I4 (Residues: 7-130; Coverage: 95%)
Gene name: CC_2463
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 36.67Å b: 40.71Å c: 66.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.206
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Getting in the loop: regulation of development in Caulobacter crescentus.
Curtis et al. (2010)
6 more

1 mention without citation

A robust bulk-solvent correction and anisotropic scaling procedure.
Afonine et al. (2005)