PDBe 1m45

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF MLC1P BOUND TO IQ2 OF MYO2P, A CLASS V MYOSIN

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin light chain 1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53141 (Residues: 2-149; Coverage: 99%)
Gene names: G3080, MLC1, YGL106W
Structure domains: EF-hand
Myosin-2 Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.99 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P19524 (Residues: 806-830; Coverage: 2%)
Gene names: CDC66, MYO2, O6167, YOR326W

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P212121
Unit cell:
a: 43.594Å b: 56.455Å c: 56.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided