PDBe 1m3x

X-ray diffraction
2.55Å resolution

Photosynthetic Reaction Center From Rhodobacter Sphaeroides

Released:
Source organism: Rhodobacter sphaeroides
Primary publication:
Interactions between lipids and bacterial reaction centers determined by protein crystallography.
Proc. Natl. Acad. Sci. U.S.A. 99 11055-60 (2002)
PMID: 12167672

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3121
Unit cell:
a: 141.8Å b: 141.8Å c: 187.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.209
Expression system: Rhodobacter sphaeroides