PDBe 1m27

X-ray diffraction
2.5Å resolution

Crystal structure of SAP/FynSH3/SLAM ternary complex

Released:
Source organism: Homo sapiens
Primary publication:
SAP couples Fyn to SLAM immune receptors.
Nat. Cell Biol. 5 155-60 (2003)
PMID: 12545174

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SH2 domain-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60880 (Residues: 1-104; Coverage: 81%)
Gene names: DSHP, SAP, SH2D1A
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein
Signaling lymphocytic activation molecule Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13291 (Residues: 276-286; Coverage: 4%)
Gene names: SLAM, SLAMF1
Tyrosine-protein kinase Fyn Chain: C
Molecule details ›
Chain: C
Length: 61 amino acids
Theoretical weight: 6.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06241 (Residues: 84-144; Coverage: 11%)
Gene name: FYN
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P41212
Unit cell:
a: 52.909Å b: 52.909Å c: 183.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.266
Expression systems:
  • Escherichia coli
  • Not provided