PDBe 1m16

X-ray diffraction
1.7Å resolution

Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag and Leu 44 Replaced with Phe (L44F), Leu 73 Replaced with Val (L73V), Val 109 Replaced with Leu (V109L) and Cys 117 Replaced with Val (C117V).

Released:
Source organism: Homo sapiens
Primary publication:
Accommodation of a highly symmetric core within a symmetric protein superfold.
Protein Sci. 12 2704-18 (2003)
PMID: 14627732

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 16-155; Coverage: 90%)
  • Best match: P05230-2 (Residues: 16-56)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2221
Unit cell:
a: 74.18Å b: 97.013Å c: 108.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.249
Expression system: Escherichia coli