PDBe 1lwy

X-ray diffraction
2.01Å resolution

hOgg1 Borohydride-Trapped Intermediate without 8-oxoguanine

Primary publication:
Product-assisted catalysis in base-excision DNA repair.
Nat. Struct. Biol. 10 204-11 (2003)
PMID: 12592398

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. 

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
N-glycosylase/DNA lyase Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O15527 (Residues: 12-327; Coverage: 92%)
Gene names: MMH, MUTM, OGG1, OGH1
Sequence domains:
Structure domains:
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: Synthetic construct
Expression system: Not provided
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.4 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6522
Unit cell:
a: 92.032Å b: 92.032Å c: 211.31Å
α: 90° β: 90° γ: 120°
R R work R free
0.222 0.222 0.258
Expression systems:
  • Escherichia coli
  • Not provided