Function and Biology

Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence

Source organism: Saccharomyces cerevisiae
Biochemical function: endonuclease activity
Biological process: protein splicing
Cellular component: not assigned

EC 7.1.2.2: H(+)-transporting two-sector ATPase

Reaction catalysed:
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Systematic name:
ATP phosphohydrolase (two-sector, H(+)-transporting)
Alternative Name(s):
  • ATP synthase
  • Bacterial Ca(2+)/Mg(2+) ATPase
  • Chloroplast ATPase
  • Coupling factors (F(o), F(1) and CF(1))
  • F(0)F(1)-ATPase
  • F(1)-ATPase
  • F(o)F(1)-ATPase
  • H(+)-transporting ATP synthase
  • H(+)-transporting ATPase
  • Mitochondrial ATPase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF05204
Domain description: Homing endonuclease
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 2 copies of Pfam domain PF05204 (Homing endonuclease) in Endonuclease PI-SceI. Showing 2 copies in chain C [auth A].

PF05203
Domain description: Hom_end-associated Hint
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 1 copy of Pfam domain PF05203 (Hom_end-associated Hint) in Endonuclease PI-SceI. Showing 1 copy in chain C [auth A].

InterPro InterPro annotations
IPR006142
Domain description: Intein
Occurring in:
  1. Endonuclease PI-SceI
IPR027434
Domain description: Homing endonuclease
Occurring in:
  1. Endonuclease PI-SceI
IPR007869
Domain description: Homing endonuclease PI-Sce
Occurring in:
  1. Endonuclease PI-SceI
IPR004042
Domain description: Intein DOD homing endonuclease
Occurring in:
  1. Endonuclease PI-SceI
IPR036844
Domain description: Hint domain superfamily
Occurring in:
  1. Endonuclease PI-SceI
IPR007868
Domain description: Hom-end-associated Hint
Occurring in:
  1. Endonuclease PI-SceI
IPR006141
Domain description: Intein N-terminal splicing region
Occurring in:
  1. Endonuclease PI-SceI
IPR003587
Domain description: Hint domain N-terminal
Occurring in:
  1. Endonuclease PI-SceI

Structure domains

CATH CATH domains
3.10.28.10
Class: Alpha Beta
Architecture: Roll
Topology: Endonuclease I-creI
Homology: Homing endonucleases
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 2 copies of CATH domain 3.10.28.10 (Endonuclease I-creI) in Endonuclease PI-SceI. Showing 2 copies in chain C [auth A].
2.170.16.10
Class: Mainly Beta
Architecture: Beta Complex
Topology: Endonuclease - Pi-scei; Chain A, domain 1
Homology: Hedgehog/Intein (Hint) domain
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 1 copy of CATH domain 2.170.16.10 (Endonuclease - Pi-scei; Chain A, domain 1) in Endonuclease PI-SceI. Showing 1 copy in chain C [auth A].
SCOP SCOP annotations
55614
Class: Alpha and beta proteins (a+b)
Fold: Homing endonuclease-like
Superfamily: Homing endonucleases
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 2 copies of SCOP domain 55614 (Intein endonuclease) in Endonuclease PI-SceI. Showing 2 copies in chain C [auth A].
51298
Class: All beta proteins
Fold: Hedgehog/intein (Hint) domain
Superfamily: Hedgehog/intein (Hint) domain
Occurring in:
  1. Endonuclease PI-SceI
The deposited structure of PDB entry 1lws contains 1 copy of SCOP domain 51298 (Intein (protein splicing domain)) in Endonuclease PI-SceI. Showing 1 copy in chain C [auth A].