PDBe 1luc

X-ray diffraction
1.5Å resolution

BACTERIAL LUCIFERASE

Released:

Function and Biology Details

Reaction catalysed:
A long-chain aldehyde + FMNH(2) + O(2) = a long-chain fatty acid + FMN + H(2)O + light. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alkanal monooxygenase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.2 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07740 (Residues: 1-355; Coverage: 100%)
Gene name: luxA
Sequence domains: Luciferase-like monooxygenase
Structure domains: FMN dependent fluorescent proteins
Alkanal monooxygenase beta chain Chain: B
Molecule details ›
Chain: B
Length: 324 amino acids
Theoretical weight: 36.38 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07739 (Residues: 1-324; Coverage: 100%)
Gene name: luxB
Sequence domains: Luciferase-like monooxygenase
Structure domains: FMN dependent fluorescent proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 150.5Å b: 59Å c: 76.5Å
α: 90° β: 93.86° γ: 90°
Expression system: Escherichia coli