PDBe 1ltq

X-ray diffraction
2.33Å resolution

CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE

Released:

Function and Biology Details

Reactions catalysed:
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA. 
A deoxyribonucleoside 3'-phosphate + H(2)O = a deoxyribonucleoside + phosphate. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polynucleotide kinase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 35.23 KDa
Source organism: Enterobacteria phage T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P06855 (Residues: 1-301; Coverage: 100%)
Gene name: pseT
Sequence domains: AAA domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I222
Unit cell:
a: 78.585Å b: 93.684Å c: 124.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.261
Expression system: Escherichia coli