PDBe 1lpd

X-ray diffraction
1.7Å resolution

HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT (COMPLEX WITH ADENINE)

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein DAP-30 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.6 KDa
Source organism: Dianthus caryophyllus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24476 (Residues: 24-277; Coverage: 94%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 37.093Å b: 78.464Å c: 42.721Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.179 0.17 0.23
Expression system: Escherichia coli