PDBe 1lo5

X-ray diffraction
3.2Å resolution

Crystal structure of the D227A variant of Staphylococcal enterotoxin A in complex with human MHC class II

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Enterotoxin type A Chain: D
Molecule details ›
Chain: D
Length: 233 amino acids
Theoretical weight: 27.09 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0L2 (Residues: 25-257; Coverage: 100%)
Gene name: entA
Sequence domains:
Structure domains:
Hemagglutinin peptide Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.51 KDa
UniProt:
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04229 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 52.008Å b: 75.792Å c: 198.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.339
Expression system: Escherichia coli