PDBe 1lf9

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D- glucose. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucoamylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 684 amino acids
Theoretical weight: 76.59 KDa
Source organism: Thermoanaerobacterium thermosaccharolyticum
UniProt:
  • Canonical: O85672 (Residues: 18-141, 142-695; Coverage: 100%)
Gene name: ga1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 80.491Å b: 102.931Å c: 164.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.227