PDBe 1lek

X-ray diffraction
2.15Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome c oxidase subunit NDUFA4 Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 1.06 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q62425 (Residues: 61-68; Coverage: 10%)
Gene name: Ndufa4
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21212
Unit cell:
a: 135.894Å b: 87.123Å c: 45.231Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.258
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)