PDBe 1ldk

X-ray diffraction
3.1Å resolution

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF Ubiquitin Ligase Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero decamer
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Cullin-1 Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 45.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: Q13616 (Residues: 15-410)
Gene name: CUL1
Cullin-1 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 42.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: Q13616 (Residues: 411-776)
Gene name: CUL1
E3 ubiquitin-protein ligase RBX1, N-terminally processed Chain: C
Molecule details ›
Chain: C
Length: 90 amino acids
Theoretical weight: 10.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P62877 (Residues: 19-108)
Gene names: RBX1, RNF75, ROC1
S-phase kinase-associated protein 2 Chain: E
Molecule details ›
Chain: E
Length: 41 amino acids
Theoretical weight: 4.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: Q13309 (Residues: 97-137)
Gene names: FBXL1, SKP2
S-phase kinase-associated protein 1 Chain: D
Molecule details ›
Chain: D
Length: 133 amino acids
Theoretical weight: 15.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P63208 (Residues: 2-140)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 219.378Å b: 50.529Å c: 158.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.289 0.289 0.331
Expression system: Escherichia coli