PDBe 1lci

X-ray diffraction
2Å resolution

FIREFLY LUCIFERASE

Released:

Function and Biology Details

Reaction catalysed:
Photinus luciferin + O(2) + ATP = oxidized Photinus luciferin + CO(2) + AMP + diphosphate + light. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.82 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P41212
Unit cell:
a: 119.53Å b: 119.53Å c: 94.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.265
Expression system: Escherichia coli