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NMR solution structure and dynamics of motilin in isotropic phospholipid bicellar solution.

Andersson A, Mäler L
J Biomol NMR 24 103-12 (2002)
Cited: 28 times
EuropePMC logo PMID: 12495026

Abstract

The structure and dynamics of the gastrointestinal peptide hormone motilin, consisting of 22 amino acid residues, have been studied in the presence of isotropic q = 0.5 phospholipid bicelles. The NMR solution structure of the peptide in acidic bicelle solution was determined from 203 NOE-derived distance constraints and six backbone torsion angle constraints. Dynamic properties for the 13Calpha-1H vector in Leu10 were determined for motilin specifically labeled with 13C at this position by analysis of multiple-field relaxation data. The structure reveals an ordered alpha-helical conformation between Glu9 and Lys20. The N-terminus is also well structured with a turn resembling that of a classical beta-turn. The 13C dynamics clearly show that motilin tumbles slowly in solution, with a correlation time characteristic of a large object. It was also found that motilin has a large degree of local flexibility as compared with what has previously been reported in SDS micelles. The results show that motilin interacts with the bicelle, displaying motional properties of a peptide bound to a membrane. In comparison, motilin in neutral bicelles seems less structured and more flexible. This study shows that the small isotropic bicelles are well suited for use as membrane-mimetic for structural as well as dynamical investigations of membrane-bound peptides by high-resolution NMR.

Reviews - 1lbj mentioned but not cited (1)

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Articles - 1lbj mentioned but not cited (1)

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Reviews citing this publication (6)

  1. Solution NMR studies of peptide-lipid interactions in model membranes. Mäler L. Mol Membr Biol 29 155-176 (2012)
  2. 3D structures of membrane-associated small molecules as determined in isotropic bicelles. Matsumori N, Murata M. Nat Prod Rep 27 1480-1492 (2010)
  3. Interpretation of biomolecular NMR spin relaxation parameters. Reddy T, Rainey JK. Biochem Cell Biol 88 131-142 (2010)
  4. Solution NMR studies of cell-penetrating peptides in model membrane systems. Mäler L. Adv Drug Deliv Rev 65 1002-1011 (2013)
  5. Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR. Liebau J, Ye W, Mäler L. Magn Reson Chem 55 395-404 (2017)
  6. The Structural Basis of Peptide Binding at Class A G Protein-Coupled Receptors. Vu O, Bender BJ, Pankewitz L, Huster D, Beck-Sickinger AG, Meiler J. Molecules 27 (2021)

Articles citing this publication (20)

  1. Helix stabilization precedes aqueous and bilayer-catalyzed fiber formation in islet amyloid polypeptide. Williamson JA, Loria JP, Miranker AD. J Mol Biol 393 383-396 (2009)
  2. Molecular dynamics simulations of the influenza hemagglutinin fusion peptide in micelles and bilayers: conformational analysis of peptide and lipids. Lagüe P, Roux B, Pastor RW. J Mol Biol 354 1129-1141 (2005)
  3. A multidimensional 1H NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles. Marcotte I, Separovic F, Auger M, Gagné SM. Biophys J 86 1587-1600 (2004)
  4. Structure and positioning comparison of two variants of penetratin in two different membrane mimicking systems by NMR. Lindberg M, Biverståhl H, Gräslund A, Mäler L. Eur J Biochem 270 3055-3063 (2003)
  5. NMR solution structure of the mitochondrial F1beta presequence from Nicotiana plumbaginifolia. Moberg P, Nilsson S, Ståhl A, Eriksson AC, Glaser E, Mäler L. J Mol Biol 336 1129-1140 (2004)
  6. Solid-state NMR spectroscopy of a membrane protein in biphenyl phospholipid bicelles with the bilayer normal parallel to the magnetic field. Park SH, Loudet C, Marassi FM, Dufourc EJ, Opella SJ. J Magn Reson 193 133-138 (2008)
  7. PFG-NMR investigations of the binding of cationic neuropeptides to anionic and zwitterionic micelles. Whitehead TL, Jones LM, Hicks RP. J Biomol Struct Dyn 21 567-576 (2004)
  8. Lipid concentration and molar ratio boundaries for the use of isotropic bicelles. Beaugrand M, Arnold AA, Hénin J, Warschawski DE, Williamson PT, Marcotte I. Langmuir 30 6162-6170 (2014)
  9. Motilin-bicelle interactions: membrane position and translational diffusion. Andersson A, Mäler L. FEBS Lett 545 139-143 (2003)
  10. Peptide design and structural characterization of a GPCR loop mimetic. Pham TC, Kriwacki RW, Parrill AL. Biopolymers 86 298-310 (2007)
  11. Conformational and molecular interaction studies of glucagon-like peptide-2 with its N-terminal extracellular receptor domain. Venneti KC, Hewage CM. FEBS Lett 585 346-352 (2011)
  12. Kinetic models for peptide-induced leakage from vesicles and cells. Andersson A, Danielsson J, Gräslund A, Mäler L. Eur Biophys J 36 621-635 (2007)
  13. Structural characterization of AS1-membrane interactions from a subset of HAMP domains. Unnerståle S, Mäler L, Draheim RR. Biochim Biophys Acta 1808 2403-2412 (2011)
  14. Maximum entropy reconstruction of joint phi, psi-distribution with a coil-library prior: the backbone conformation of the peptide hormone motilin in aqueous solution from phi and psi-dependent J-couplings. Massad T, Jarvet J, Tanner R, Tomson K, Smirnova J, Palumaa P, Sugai M, Kohno T, Vanatalu K, Damberg P. J Biomol NMR 38 107-123 (2007)
  15. The NMR-derived conformation of orexin-A: an orphan G-protein coupled receptor agonist involved in appetite regulation and sleep. Miskolzie M, Kotovych G. J Biomol Struct Dyn 21 201-210 (2003)
  16. Association between metabolic profile and microbiomic changes in rats with functional dyspepsia. Luo L, Hu M, Li Y, Chen Y, Zhang S, Chen J, Wang Y, Lu B, Xie Z, Liao Q. RSC Adv 8 20166-20181 (2018)
  17. Thermodynamics of partitioning of substance P in isotropic bicelles. Kim C, Baek SB, Kim DH, Lim SC, Lee HJ, Lee HC. J Pept Sci 15 353-358 (2009)
  18. Binding and folding of melittin in the presence of ganglioside GM1 micelles. Chatterjee C, Mukhopadhyay C. J Biomol Struct Dyn 23 183-192 (2005)
  19. Capability of ganglioside GM1 in modulating interactions, structure, location and dynamics of peptides/proteins: biophysical approaches: interaction of ganglioside GM1 with peptides/proteins. Khatun UL, Gayen A, Mukhopadhyay C. Glycoconj J 31 435-447 (2014)
  20. Refining internal bilayer structure of bicelles resolved by extended-q small angle X-ray scattering. Cheu C, Yang L, Nieh MP. Chem Phys Lipids 231 104945 (2020)


Related citations provided by authors (1)

  1. Three-dimensional structure and position of porcine motilin in sodium dodecyl sulfate micelles determined by 1H NMR.. Jarvet J, Zdunek J, Damberg P, Gräslund A Biochemistry 36 8153-63 (1997)

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