PDBe 1lat

X-ray diffraction
1.9Å resolution

GLUCOCORTICOID RECEPTOR MUTANT/DNA COMPLEX

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.37 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06536 (Residues: 440-515; Coverage: 10%)
Gene names: Grl, Nr3c1
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*TP*TP*CP*CP*AP*GP*AP*AP*CP*AP*TP*GP*TP*TP*CP*TP*G P*GP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 38.72Å b: 76.07Å c: 118.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided