PDBe 1l8t

X-ray diffraction
2.4Å resolution

Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Kanamycin A Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.88 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P0A3Y5 (Residues: 2-264)
Gene name: aphA

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P4322
Unit cell:
a: 46.629Å b: 46.629Å c: 301.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.291
Expression system: Escherichia coli BL21(DE3)