PDBe 1l7v

X-ray diffraction
3.2Å resolution

Bacterial ABC Transporter Involved in B12 Uptake

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vitamin B12 import ATP-binding protein BtuD Chains: C, D
Molecule details ›
Chains: C, D
Length: 249 amino acids
Theoretical weight: 27.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06611 (Residues: 1-249; Coverage: 100%)
Gene names: JW1699, b1709, btuD
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Vitamin B12 import system permease protein BtuC Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06609 (Residues: 1-326; Coverage: 100%)
Gene names: JW1701, b1711, btuC
Sequence domains: FecCD transport family
Structure domains: ABC transporter involved in vitamin B12 uptake, BtuC

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 92.99Å b: 106.292Å c: 95.543Å
α: 90° β: 99.29° γ: 90°
R-values:
R R work R free
0.262 0.262 0.286
Expression system: Escherichia coli