› 1l5z

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE E121K SUBSTITUTION OF THE RECEIVER DOMAIN OF SINORHIZOBIUM MELILOTI DCTD

Released:
DOI: 10.2210/pdb1l5z/pdb
PDB entry 1l5z coloured by chain, front view.
PDB entry 1l5z coloured by chain, side view.
PDB entry 1l5z coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Primary publication:
Two-component signaling in the AAA + ATPase DctD: binding Mg2+ and BeF3- selects between alternate dimeric states of the receiver domain.
Park S, Meyer M, Jones AD, Yennawar HP, Yennawar NH, Nixon BT
FASEB J 16 1964-6 (2002)
PMID: 12368235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C4-dicarboxylate transport transcriptional regulatory protein DctD Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 16.82 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli
UniProt:
  • Canonical: P13632 (Residues: 2-143; Coverage: 31%)
Gene names: RB1525, SMb20613, dctD
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 57.129Å b: 58.139Å c: 168.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial response regulators: versatile regulatory strategies from common domains.
Gao et al. (2007)
4 more

1 mention without citation

Sequence signatures of allosteric proteins towards rational design.
Namboodiri et al. (2010)