PDBe 1l3r

X-ray diffraction
2Å resolution

Crystal Structure of a Transition State Mimic of the Catalytic Subunit of cAMP-dependent Protein Kinase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.2 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P63249 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 72.68Å b: 75.7Å c: 80.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.232
Expression systems:
  • Escherichia coli
  • Not provided