› 1l2f

X-ray diffraction
2.5Å resolution

Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain

Released:
DOI: 10.2210/pdb1l2f/pdb
PDB entry 1l2f coloured by chain, front view.
PDB entry 1l2f coloured by chain, side view.
PDB entry 1l2f coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain.
Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH
Biochemistry 42 13429-37 (2003)
PMID: 14621988

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.15 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X298 (Residues: 1-344; Coverage: 100%)
Gene names: TM_1777, nusA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 116.191Å b: 116.191Å c: 64.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.223 0.298
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Bacterial transcription elongation factors: new insights into molecular mechanism of action.
Borukhov et al. (2005)
2 more

9 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
8 more