PDBe 1l1r

X-ray diffraction
1.95Å resolution

Crystal Structure of APRTase from Giardia lamblia Complexed with 9-deazaadenine, Mg2+ and PRPP

Released:

Function and Biology Details

Reaction catalysed:
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.29 KDa
Source organism: Giardia intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q967M2 (Residues: 1-180; Coverage: 100%)
Gene name: DHA2_112885
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P3121
Unit cell:
a: 54.325Å b: 54.325Å c: 108.744Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.218 0.262
Expression system: Escherichia coli