PDBe 1l0o

X-ray diffraction
2.9Å resolution

Crystal Structure of the Bacillus stearothermophilus Anti-Sigma Factor SpoIIAB with the Sporulation Sigma Factor SigmaF

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA polymerase sigma factor Chain: C
Molecule details ›
Chain: C
Length: 243 amino acids
Theoretical weight: 27.94 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: O32728 (Residues: 7-245; Coverage: 96%)
Sequence domains:
Structure domains: "winged helix" repressor DNA binding domain
Anti-sigma F factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.77 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: O32727 (Residues: 1-142; Coverage: 97%)
Gene name: spoIIAB
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Heat Shock Protein 90

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 97.31Å b: 97.31Å c: 262.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.279
Expression system: Escherichia coli