PDBe 1kzl

X-ray diffraction
2.1Å resolution

Riboflavin Synthase from S.pombe bound to Carboxyethyllumazine

Released:

Function and Biology Details

Reaction catalysed:
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D- ribitylamino)-5-amino-2,6-dihydroxypyrimidine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin synthase Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.89 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y7P0 (Residues: 1-208; Coverage: 100%)
Gene names: SPCC1450.13c, rib5
Sequence domains: Lumazine binding domain
Structure domains: Elongation Factor Tu (Ef-tu); domain 3

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P61
Unit cell:
a: 70.485Å b: 70.485Å c: 92.952Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.185 0.22
Expression system: Escherichia coli