PDBe 1kyq

X-ray diffraction
2.2Å resolution

Met8p: A bifunctional NAD-dependent dehydrogenase and ferrochelatase involved in siroheme synthesis.


Function and Biology Details

Reactions catalysed:
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+). 
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Siroheme biosynthesis protein MET8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 274 amino acids
Theoretical weight: 32.29 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P15807 (Residues: 1-274; Coverage: 100%)
Gene names: MET8, YBR1461, YBR213W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 156.988Å b: 80.856Å c: 103.975Å
α: 90° β: 121.88° γ: 90°
R R work R free
0.22 0.219 0.283
Expression system: Escherichia coli