› 1kya

X-ray diffraction
2.4Å resolution

ACTIVE LACCASE FROM TRAMETES VERSICOLOR COMPLEXED WITH 2,5-XYLIDINE

Released:
DOI: 10.2210/pdb1kya/pdb
PDB entry 1kya coloured by chain, front view.
PDB entry 1kya coloured by chain, side view.
PDB entry 1kya coloured by chain, top view.
Source organism: Trametes versicolor
Primary publication:
Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics.
Bertrand T, Jolivalt C, Briozzo P, Caminade E, Joly N, Madzak C, Mougin C
Biochemistry 41 7325-33 (2002)
PMID: 12044164

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 499 amino acids
Theoretical weight: 53.37 KDa
Source organism: Trametes versicolor
UniProt:
  • Canonical: Q96UT7 (Residues: 22-520; Coverage: 100%)
Gene name: lac1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand NAG 27 x NAG
Ligand CU 16 x CU
Ligand PYE 9 x PYE
Ligand XYD 4 x XYD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 87.72Å b: 110.52Å c: 123.201Å
α: 90° β: 103.436° γ: 90°
R-values:
R R work R free
0.253 0.253 0.276

Quick links

Citations

28 review citations

Copper active sites in biology.
Solomon et al. (2014)
27 more

40 mentions without citation

Laccases: structure, function, and potential application in water bioremediation.
Arregui et al. (2019)
39 more