PDBe 1kxt

X-ray diffraction
2Å resolution

Camelid VHH Domains in Complex with Porcine Pancreatic alpha-Amylase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pancreatic alpha-amylase Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 496 amino acids
Theoretical weight: 55.46 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00690 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2
Sequence domains:
Structure domains:
IMMUNOGLOBULIN VHH FRAGMENT Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 52.78Å b: 286.85Å c: 65.98Å
α: 90° β: 93.73° γ: 90°
R-values:
R R work R free
0.203 0.203 0.236
Expression system: Escherichia coli