› 1kxm

X-ray diffraction
1.74Å resolution

Crystal structure of Cytochrome c Peroxidase with a Proposed Electron Transfer Pathway Excised to Form a Ligand Binding Channel.

Released:
DOI: 10.2210/pdb1kxm/pdb
PDB entry 1kxm coloured by chain, front view.
PDB entry 1kxm coloured by chain, side view.
PDB entry 1kxm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Excision of a proposed electron transfer pathway in cytochrome c peroxidase and its replacement by a ligand-binding channel.
Rosenfeld RJ, Hays AM, Musah RA, Goodin DB
Protein Sci 11 1251-9 (2002)
PMID: 11967381

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00431 (Residues: 71-361; Coverage: 80%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand BZI 1 x BZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 106.5Å b: 75Å c: 50.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Predicting Binding Free Energies: Frontiers and Benchmarks.
Mobley et al. (2017)
2 more

4 mentions without citation

Blind prediction of charged ligand binding affinities in a model binding site.
Rocklin et al. (2013)
3 more