PDBe 1kxh

X-ray diffraction
2.3Å resolution

Crystal structure of the complex between an inactive mutant of psychrophilic alpha-amylase (D174N) and acarbose

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 48.95 KDa
Source organism: Pseudoalteromonas haloplanktis
Expression system: Escherichia coli
UniProt:
  • Best match: P29957 (Residues: 25-472)
Gene name: amy

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: C2221
Unit cell:
a: 71.1Å b: 139.9Å c: 115.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.138 0.188
Expression system: Escherichia coli