PDBe 1kwb

X-ray diffraction
2Å resolution

Crystal structure of the His145Ala mutant of 2,3-dihydroxybipheny dioxygenase (BphC)

Released:

Function and Biology Details

Reaction catalysed:
Biphenyl-2,3-diol + O(2) = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biphenyl-2,3-diol 1,2-dioxygenase Chain: B
Molecule details ›
Chain: B
Length: 292 amino acids
Theoretical weight: 32.08 KDa
Source organism: Pseudomonas sp. KKS102
Expression system: Escherichia coli
UniProt:
  • Canonical: P17297 (Residues: 2-293; Coverage: 100%)
Gene name: bphC
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: I422
Unit cell:
a: 121.65Å b: 121.65Å c: 109.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.175
Expression system: Escherichia coli