› 1kv7

X-ray diffraction
1.4Å resolution

Crystal Structure of CueO, a multi-copper oxidase from E. coli involved in copper homeostasis

Released:
DOI: 10.2210/pdb1kv7/pdb
PDB entry 1kv7 coloured by chain, front view.
PDB entry 1kv7 coloured by chain, side view.
PDB entry 1kv7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure and electron transfer kinetics of CueO, a multicopper oxidase required for copper homeostasis in Escherichia coli.
Roberts SA, Weichsel A, Grass G, Thakali K, Hazzard JT, Tollin G, Rensing C, Montfort WR
Proc Natl Acad Sci U S A 99 2766-71 (2002)
PMID: 11867755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 53.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand C2O 1 x C2O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 49.82Å b: 90.51Å c: 53.09Å
α: 90° β: 102.9° γ: 90°
R-values:
R R work R free
0.185 0.185 0.221
Expression system: Escherichia coli BL21

Quick links

Citations

27 review citations

Copper active sites in biology.
Solomon et al. (2014)
26 more

21 mentions without citation

Resistance mechanisms of Mycobacterium tuberculosis against phagosomal copper overload.
Rowland et al. (2012)
20 more