› 1ksw

X-ray diffraction
2.8Å resolution

Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP

Released:
DOI: 10.2210/pdb1ksw/pdb
PDB entry 1ksw coloured by chain, front view.
PDB entry 1ksw coloured by chain, side view.
PDB entry 1ksw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme.
Witucki LA, Huang X, Shah K, Liu Y, Kyin S, Eck MJ, Shokat KM
Chem Biol 9 25-33 (2002)
PMID: 11841936

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 51.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12931 (Residues: 86-536; Coverage: 84%)
Gene names: SRC, SRC1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NBS 1 x NBS
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 50.81Å b: 87.68Å c: 106.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.294
Expression system: Spodoptera frugiperda

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Citations

10 review citations

RNA polymerase II C-terminal domain: Tethering transcription to transcript and template.
Corden JL. (2013)
9 more

11 mentions without citation

Understanding and exploiting substrate recognition by protein kinases.
Turk BE. (2008)
10 more