PDBe 1ksj

X-ray diffraction
2.6Å resolution

Complex of Arl2 and PDE delta, Crystal Form 2 (SeMet)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor-like protein 2 Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D0J4 (Residues: 1-184; Coverage: 100%)
Gene name: Arl2
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 17.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43924 (Residues: 1-150; Coverage: 100%)
Gene names: PDE6D, PDED
Sequence domains: GMP-PDE, delta subunit
Structure domains: GMP phosphodiesterase, delta subunit

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P212121
Unit cell:
a: 44.815Å b: 65.726Å c: 103.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.209 0.264
Expression system: Escherichia coli