PDBe 1kra

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF KLEBSIELLA AEROGENES UREASE, ITS APOENZYME AND TWO ACTIVE SITE MUTANTS

Released:
Source organism: Klebsiella aerogenes
Primary publication:
Structures of the Klebsiella aerogenes urease apoenzyme and two active-site mutants.
Biochemistry 35 10616-26 (1996)
PMID: 8718850

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.1 KDa
Source organism: Klebsiella aerogenes
UniProt:
  • Canonical: P18316 (Residues: 1-100; Coverage: 100%)
Gene name: ureA
Sequence domains: Urease, gamma subunit
Structure domains: Urease, gamma-subunit
Urease subunit beta Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 11.71 KDa
Source organism: Klebsiella aerogenes
UniProt:
  • Canonical: P18315 (Residues: 1-106; Coverage: 100%)
Gene name: ureB
Sequence domains: Urease beta subunit
Structure domains: Urease, beta-subunit
Urease subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 567 amino acids
Theoretical weight: 60.37 KDa
Source organism: Klebsiella aerogenes
UniProt:
  • Canonical: P18314 (Residues: 1-567; Coverage: 100%)
Gene name: ureC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I213
Unit cell:
a: 170.8Å b: 170.8Å c: 170.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 not available