PDBe 1klj

X-ray diffraction
2.44Å resolution

Crystal structure of uninhibited factor VIIa

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 69 amino acids
Theoretical weight: 7.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08709 (Residues: 144-212; Coverage: 16%)
Gene name: F7
Structure domains: Laminin
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P41212
Unit cell:
a: 94.443Å b: 94.443Å c: 114.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.286
Expression system: Escherichia coli BL21