PDBe 1kkl

X-ray diffraction
2.8Å resolution

L.casei HprK/P in complex with B.subtilis HPr

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HPr kinase/phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 205 amino acids
Theoretical weight: 22.68 KDa
Source organism: Lactobacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RE09 (Residues: 128-319; Coverage: 60%)
Gene name: hprK
Sequence domains: HPr Serine kinase C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Phosphocarrier protein HPr Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 100 amino acids
Theoretical weight: 10.7 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08877 (Residues: 1-88; Coverage: 100%)
Gene names: BSU13900, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: Histidine-containing Protein; Chain: A;

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3212
Unit cell:
a: 81.135Å b: 81.135Å c: 253.192Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.264
Expression system: Escherichia coli