PDBe 1kfu

X-ray diffraction
2.5Å resolution

Crystal Structure of Human m-Calpain Form II

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calpain-2 catalytic subunit Chain: L
Molecule details ›
Chain: L
Length: 699 amino acids
Theoretical weight: 79.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P17655 (Residues: 2-700; Coverage: 100%)
  • Best match: P17655-2 (Residues: 2-622)
Gene names: CANPL2, CAPN2
Sequence domains:
Structure domains:
Calpain small subunit 1 Chain: S
Molecule details ›
Chain: S
Length: 184 amino acids
Theoretical weight: 21.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04632 (Residues: 85-268; Coverage: 69%)
Gene names: CAPN4, CAPNS, CAPNS1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 51.88Å b: 169.84Å c: 64.44Å
α: 90° β: 95.12° γ: 90°
R-values:
R R work R free
0.221 0.221 0.276
Expression system: Spodoptera frugiperda